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PCR And Its Use Beginning of Paper Often times, scientists only have a small amount of DNA to deal with when doing genetic research or studies. In these situations, scientists can do one of several things. One is to just try to work with it anyway, but this is nearly impossible (depending on how much there is). The .... Middle of Paper .... the primer, etc). Then they have to run it through the PCR machine. Here is a (rather detailed) description of the process: "The cycling protocol consisted of 25-30 cycles of three- temperatures: strand denaturation at 95degC, primer annealing at 55degC, and primer extension at 72deg C, typically 30 seconds, 30 seconds, and 60 seconds for the DNA Thermal Cycler and 4 seconds, 10 seconds, and 60 seconds for the Thermal Cycler 9600, respectively." Basically, that means that they set it to certain temperatures, then put it in different .... 773 words | 3 pages Subscription Length Price Join 30 days membership (recurring) $19.95 90 days membership (recurring) $39.95 180 days membership (non-recurring) $69.95