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Bacteria Classification By Gram Staining Beginning of Paper THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high .... Middle of Paper .... c. 95% Ethyl Alcohol d. Safranin e. Distilled Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil Procedure: A. Preparation : 1. Bacteria is cultivated on agar jelly in an incubator at 25°C for 24 hours. 2. Obtain a microscope slide and with a toothpick, smear a thin coat of the bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet and leave standing for 20 seconds 4. Wash off the stain with distilled water; drain and blot off the excess with bibulous paper. 5. Apply Gram’s Iodine on the smear and .... 751 words | 3 pages Subscription Length Price Join 30 days membership (recurring) $19.95 90 days membership (recurring) $39.95 180 days membership (non-recurring) $69.95